Team:TUDelft/14 August 2009
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+ | ===Tim Weenink=== | ||
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+ | First did ligation of !E and !F with the parts that were not excised from gel. | ||
+ | |||
+ | {| border="1" align="center" | ||
+ | | Component for !E || volume (uL) | ||
+ | |- align="center" | ||
+ | | *S PCR (upstream) || 2 | ||
+ | |- align="center" | ||
+ | | !C PCR (downstream) || 2 | ||
+ | |- align="center" | ||
+ | | pSB1AK3 from !A assembly || 2 | ||
+ | |- align="center" | ||
+ | | T4 ligase buffer || 2 | ||
+ | |- align="center" | ||
+ | | T4 ligase || 1 | ||
+ | |- align="center" | ||
+ | | H2O || 11 | ||
+ | |} | ||
+ | |||
+ | {| border="1" align="center" | ||
+ | | Component for !F || volume (uL) | ||
+ | |- align="center" | ||
+ | | !B PCR (upstream) || 2 | ||
+ | |- align="center" | ||
+ | | !D PCR (downstream) || 2 | ||
+ | |- align="center" | ||
+ | | pSB1AK3 from !A assembly || 2 | ||
+ | |- align="center" | ||
+ | | T4 ligase buffer || 2 | ||
+ | |- align="center" | ||
+ | | T4 ligase || 1 | ||
+ | |- align="center" | ||
+ | | H2O || 11 | ||
+ | |} | ||
+ | |||
+ | |||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} |
Revision as of 12:47, 14 August 2009
Lab Notebook
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14 August 2009
Tim Weenink
First did ligation of !E and !F with the parts that were not excised from gel.
Component for !E | volume (uL) |
*S PCR (upstream) | 2 |
!C PCR (downstream) | 2 |
pSB1AK3 from !A assembly | 2 |
T4 ligase buffer | 2 |
T4 ligase | 1 |
H2O | 11 |
Component for !F | volume (uL) |
!B PCR (upstream) | 2 |
!D PCR (downstream) | 2 |
pSB1AK3 from !A assembly | 2 |
T4 ligase buffer | 2 |
T4 ligase | 1 |
H2O | 11 |