Team:TUDelft/20 August 2009

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(Difference between revisions)
(→Tim Weenink)
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*!SIIE1C3ß2
*!SIIE1C3ß2
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===Daniel===
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Growth in assemblies 1A, 2A, 3A, 4A and 5A. 6A failbut we found a biobrick with the same structure and we have it in plates already!!
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Miniprep of succeed assemblies:
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{| border="1" align="center"
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| Assembly || [DNA] ng/uL
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|- align="center"
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| 1A || 20.5
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|- align="center"
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| 2A I || 18.7
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|- align="center"
 +
| 2A II || 26.5
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|- align="center"
 +
| 2A III || 25.5
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|- align="center"
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| 2A IV || 32.2
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|- align="center"
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| 3A || 13
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|- align="center"
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| 4A I || 13.9
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|- align="center"
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| 4A II || 8.7
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|- align="center"
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| 5A || 4.9
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|} 
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 +
Meeting with Groningen's team (see pictures)
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 16:22, 29 August 2009

Lab Notebook

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20 August 2009

Tim Weenink

We got colonies on yesterdays transformations. RbCl plates had about 20 colonies each, while Electrocompetent cells were hundreds of colonies. Finally an oldfashioned highly efficient transformation. I suspect transformations with larger parts (>1kb) work better.

I inoculated some of the colonies. These are the names of the samples:

R/E stands for RbCl/Electrocompetent (resp) 1C3 stands for the used backbone (pSB1C3) α ß stands for plate numbers 1,2 stands for colony numbers

  • !SIIR1C3α1
  • !SIIR1C3α2
  • !SIIR1C3ß1
  • !SIIR1C3ß2
  • !SIIE1C3α1
  • !SIIE1C3α2
  • !SIIE1C3ß1
  • !SIIE1C3ß2

Daniel

Growth in assemblies 1A, 2A, 3A, 4A and 5A. 6A failbut we found a biobrick with the same structure and we have it in plates already!!

Miniprep of succeed assemblies:

Assembly [DNA] ng/uL
1A 20.5
2A I 18.7
2A II 26.5
2A III 25.5
2A IV 32.2
3A 13
4A I 13.9
4A II 8.7
5A 4.9

Meeting with Groningen's team (see pictures)

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