Team:TUDelft/24 August 2009

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(New page: Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies)
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{{Template:TUDelftiGEM2009}}
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{{Template:TUDelftiGEM2009_LabNotebook}}
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='''24 August 2009'''=
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Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies
Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies
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===Daniel===
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Digestion of assemblies in order to do the second round of assembly.
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Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR
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[[Image:Gel240809.jpg|600px]]
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'''Right Gel (2%)'''
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{| border="1" align="center"
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| Well || Assembly || Expected Plasmid Size || Status
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|- align="center"
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| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 2 || 4A || 824  || ?
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|- align="center"
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| 3 || 5A || 1040 || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 4 || 2A || 214  || ?
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|- align="center"
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| 5 || 3A || 183 || ?
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|- align="center"
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| 6 || 1A || 1076 || ?
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|- align="center"
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| 7 || PCR 1 ||  ||
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|- align="center"
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| 8 || PCR 2 ||  ||
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|- align="center"
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| 9 || PCR 3 ||  ||
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|- align="center"
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| 10 || PCR 4 ||  ||
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|- align="center"
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| 11 || PCR 5 ||  ||
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|- align="center"
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| 12 || PCR 6 ||  ||
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|}
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{{Template:TUDelftiGEM2009_end}}

Revision as of 16:45, 29 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

24 August 2009

Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies

Daniel

Digestion of assemblies in order to do the second round of assembly. Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR

Gel240809.jpg

Right Gel (2%)

Well Assembly Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
2 4A 824  ?
3 5A 1040 ✔
4 2A 214  ?
5 3A 183  ?
6 1A 1076  ?
7 PCR 1
8 PCR 2
9 PCR 3
10 PCR 4
11 PCR 5
12 PCR 6

Retrieved from "http://2009.igem.org/Team:TUDelft/24_August_2009"