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Team:TUDelft/2 September 2009
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Tim Weenink (Talk | contribs) (New page: {{Template:TUDelftiGEM2009}} {{Template:TUDelftiGEM2009_LabNotebook}} ='''2 September 2009'''= ===Tim Weenink=== Ligation of !SIII: placI upstream, *T1 (I-SceI) downstream, pSB1C3 backb...) |
Tim Weenink (Talk | contribs) (→Tim Weenink) |
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also did a gel to check all the above. | also did a gel to check all the above. | ||
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Revision as of 16:34, 2 September 2009
Lab Notebook
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2 September 2009
Tim Weenink
Ligation of !SIII: placI upstream, *T1 (I-SceI) downstream, pSB1C3 backbone. Also did yesterdays ligation (!P) again, because the PCR machine was set to a ligation temp of 37 degrees, where it should be 20.
I inoculated the K12 strain in liquid culture for preparation of competent cells tomorrow.
also did a gel to check all the above.
