Team:TUDelft/8 September 2009

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(New page: {{Template:TUDelftiGEM2009}} {{Template:TUDelftiGEM2009_LabNotebook}} ='''8 September 2009'''= ===Tim Weenink=== Today: Plasmid isolation: out of 10 !SIII inoculated cultures, only 3,5 ...)
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out of 10 !SIII inoculated cultures, only 3,5 and 6 showed proper growth. The others showed very low growth and will be incubated for more time. Growth/low growth is not related to colony size, which seemed to have two growing modes: big colonies and small colonies.  
out of 10 !SIII inoculated cultures, only 3,5 and 6 showed proper growth. The others showed very low growth and will be incubated for more time. Growth/low growth is not related to colony size, which seemed to have two growing modes: big colonies and small colonies.  
 +
Did miniprep and made glycerol stock and did E-P restriction of the following:
 +
 +
{| border="1"
 +
| part || concentration || 260/280 || 260/230
 +
|-
 +
| !SIII3 || 100.1    || 2.00 || 2.27
 +
|-
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| !SIII4 || 3.3    || 2.94 || 2.94
 +
|-
 +
| !SIII5 || 102.4 || 1.93 || 2.28
 +
|-
 +
| !SIII6 || 117.2 || 1.96 || 2.24
 +
|}
 +
 +
Also did E-P restriction of the pSB4C5 backbone. This was all gelled:
 +
 +
 +
Finally I did a ligation of pLacI (upstream), *T1 (BBa_K142202: I-SceI homing endonuclease with RBS & double terminator) (downstream) and pSB4C5 as a backbone.
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Revision as of 10:11, 13 September 2009

Lab Notebook

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8 September 2009

Tim Weenink

Today: Plasmid isolation: out of 10 !SIII inoculated cultures, only 3,5 and 6 showed proper growth. The others showed very low growth and will be incubated for more time. Growth/low growth is not related to colony size, which seemed to have two growing modes: big colonies and small colonies.

Did miniprep and made glycerol stock and did E-P restriction of the following:

part concentration 260/280 260/230
 !SIII3 100.1 2.00 2.27
 !SIII4 3.3 2.94 2.94
 !SIII5 102.4 1.93 2.28
 !SIII6 117.2 1.96 2.24

Also did E-P restriction of the pSB4C5 backbone. This was all gelled:


Finally I did a ligation of pLacI (upstream), *T1 (BBa_K142202: I-SceI homing endonuclease with RBS & double terminator) (downstream) and pSB4C5 as a backbone.


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