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Team:TUDelft/15 September 2009
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| + | None of yesterdays inoculations showed GFP expression. All aTc induced inoculations should have. So I did PCR reactions to check the lengths of the inserts. | ||
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| + | These reactions were gelled: | ||
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| + | After that I did replicaplating of all !Z plates on plates with Cam and Cam&aTc. This way I could screen for colonies that did express GFP when induced with aTc. But it later turned out that none of the colonies on the plate with aTc expressed GFP. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 11:38, 27 September 2009
Lab Notebook
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15 September 2009
Tim Weenink
None of yesterdays inoculations showed GFP expression. All aTc induced inoculations should have. So I did PCR reactions to check the lengths of the inserts.
These reactions were gelled:
After that I did replicaplating of all !Z plates on plates with Cam and Cam&aTc. This way I could screen for colonies that did express GFP when induced with aTc. But it later turned out that none of the colonies on the plate with aTc expressed GFP.
