Team:UNICAMP-Brazil/Notebooks/September 29

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''Raíssa and Taís''
''Raíssa and Taís''
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==''' ColiGuard '''==
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====Miniprep Results====
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* We ran an agarose gel with the minipreps samples (proccedure performed yesterday). We successful recovered all plasmids (BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I718016).
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''Marcelo''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 18:23, 6 October 2009

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YeastGuard

Dephosphorylation - CIAP test

  • Only a few colonies were found this morning =(. Unfortunately the number of colonies wasn´t enough to compare and decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.

New biobricks

  • Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).

  • We transformed the thermocompetent E. coli (Protocol 3) with the ligations and plated in LB+Amp media.


Raíssa and Taís


ColiGuard

Miniprep Results

  • We ran an agarose gel with the minipreps samples (proccedure performed yesterday). We successful recovered all plasmids (BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I718016).

Marcelo




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