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Team:Paris/Production overview
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Normally, bacteria make vesicles to infect other cells. In our project, we want to do more vesicles to develop a long distance communication system between gram-negative bacteria. So we need to destabilize the outer membrane to create outer membrane vesicles (OMVs). We found that the best method is to use the Tol/Pal system<sup><span id="1">[[Team:Paris/Production_overview#References|[1]]]</span></sup>. | Normally, bacteria make vesicles to infect other cells. In our project, we want to do more vesicles to develop a long distance communication system between gram-negative bacteria. So we need to destabilize the outer membrane to create outer membrane vesicles (OMVs). We found that the best method is to use the Tol/Pal system<sup><span id="1">[[Team:Paris/Production_overview#References|[1]]]</span></sup>. | ||
The Tol/Pal system of ''Escherichia Coli'' is required to maintain outer membrane integrity. It is composed of five cell envelope proteins, TolA, TolB, TolQ, TolR and Pal,included in two complexs..<sup><span id="2">[[Team:Paris/Production_overview#References|[2]]]</span></sup><sup><span id="3">[[Team:Paris/Production_overview#References|[3]]]</span></sup> | The Tol/Pal system of ''Escherichia Coli'' is required to maintain outer membrane integrity. It is composed of five cell envelope proteins, TolA, TolB, TolQ, TolR and Pal,included in two complexs..<sup><span id="2">[[Team:Paris/Production_overview#References|[2]]]</span></sup><sup><span id="3">[[Team:Paris/Production_overview#References|[3]]]</span></sup> | ||
Revision as of 21:52, 14 October 2009
iGEM > Paris > Vesicle production system > Overview
Introduction
Normally, bacteria make vesicles to infect other cells. In our project, we want to do more vesicles to develop a long distance communication system between gram-negative bacteria. So we need to destabilize the outer membrane to create outer membrane vesicles (OMVs). We found that the best method is to use the Tol/Pal system[1]. The Tol/Pal system of Escherichia Coli is required to maintain outer membrane integrity. It is composed of five cell envelope proteins, TolA, TolB, TolQ, TolR and Pal,included in two complexs..[2][3]
See Tol/Pal part for more information and why we choose this system. Strategy and Construction part explains our aim in this OMV production system.
References
- ^Transmembrane a-helix interactions are required for the functional assembly of the escherichia coli Tol complex. Lazzaroni & Geli 1995 - [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179564 179564]
- ^The Tol-Pal proteins of the Escherichia coli cell envelope an energized system required for outer membrane integrity. Lloubès & Journet 2001 - [http://www.ncbi.nlm.nih.gov/pubmed/11501670 11501670]
- ^Improved methods for producing outer membrane vesicles in gram-negative bacteria. Henry & Lloubès 2004 - [http://www.ncbi.nlm.nih.gov/pubmed/15249060 15249060]
