Lab Notebook
29 July 2009
Calin
Checked concentrations from yesterday's miniprep.
Part | Concentration (ng/uL)
|
pSB1AK3 I | 92
|
pSB1AK3 II | 77
|
lambda_p GFP I | 119
|
lambda_p GFP II | 108
|
pLacI | 56.7
|
RBS | 95.2
|
Todays gel:
Well | Part | Expected Size
|
1 | BBa_K142205 PCR |
|
2 | BBa_K142202 PCR |
|
3 | BBa_B0015 PCR |
|
4 | BBa_R0040 PCR |
|
5 | BBa_K145201 PCR |
|
6 | *I3 uncut |
|
7 | *I3 BglI cut |
|
8 | *I3 BglI+I-SceI cut |
|
9 | *I6 uncut |
|
10 | *I6 BglI cut |
|
11 | *I7 BglI+I-SceI cut |
|
12 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] |
|
13 | |
|
14 | |
|
15 | |
|
16 | |
|
17 | |
|
18 | |
|
19 | |
|
20 | |
|
Orr
Got the source code in R for the modelling of conjugation, and already looked at how it works.