Team:Paris/Freezer Primers
From 2009.igem.org
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
Color code for sticker | |||||||
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Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Primers
[O1] Rev P1 | |||||
---|---|---|---|---|---|
Description | tolR | ||||
Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG | ||||
Designer | D. Bikard | ||||
Worked |
[O2] Fwd P2 | |||||
---|---|---|---|---|---|
Description | tolR (DO NOT USE ERROR) | ||||
Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG | ||||
Designer | D. Bikard | ||||
Worked |
[O3] Rev P3 | |||||
---|---|---|---|---|---|
Description | tolA | ||||
Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG | ||||
Designer | D. Bikard | ||||
Worked |
[O4] Rev P4 | |||||
---|---|---|---|---|---|
Description | tolA (ERROR: Not designed for N-term fusion) | ||||
Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC | ||||
Designer | D. Bikard | ||||
Worked |
[O5] Rev P5 | |||||
---|---|---|---|---|---|
Description | tatABC | ||||
Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC | ||||
Designer | D. Bikard | ||||
Worked |
[06] Fwd P6 | |||||
---|---|---|---|---|---|
Description | tatABC (without RBS) | ||||
Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG | ||||
Designer | D. Bikard | ||||
Worked |
[O7] Rev P7 | |||||
---|---|---|---|---|---|
Description | clyA fusion | ||||
Sequence (5' > 3') | TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC | ||||
Designer | D. Bikard | ||||
Worked |
[O8] Fwd P8 | |||||
---|---|---|---|---|---|
Description | clyA fusion (ERROR: No poly-Gly linker) | ||||
Sequence (5' > 3') | TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA | ||||
Designer | D. Bikard | ||||
Worked |
[O9] clyA_Rw | |||||
---|---|---|---|---|---|
Description | clyA | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O10] clyA_fusion_polyG_Rw | |||||
---|---|---|---|---|---|
Description | clyA fusion (with 5-Gly linker) | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O11] ompA_signal_Fw | |||||
---|---|---|---|---|---|
Description | ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS | ||||
Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O12] ompA_signal_fusion_Rw | |||||
---|---|---|---|---|---|
Description | ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O13] tolR_II_Fusion_Fw | |||||
---|---|---|---|---|---|
Description | For fusion of tolR domain II (standard 23) | ||||
Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O14] tatABC_RBS_Fw | |||||
---|---|---|---|---|---|
Description | Amplification from tatA - contains RBS | ||||
Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O15] TE3_fusion_Fw | |||||
---|---|---|---|---|---|
Description | Fusion of N-term domain of colicin E3 (standard 23) | ||||
Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O16] TE3_Rw | |||||
---|---|---|---|---|---|
Description | Amplification of N-term domain of colicin E3 (standard 23) | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O17] Tg3p_Rw | |||||
---|---|---|---|---|---|
Description | Amplification of N-term domain of M13's g3p | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O18] Tg3p_fusion_Fw | |||||
---|---|---|---|---|---|
Description | Fusion of N-term domain of M13's g3p (standard 23) | ||||
Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O19] g3p_F | |||||
---|---|---|---|---|---|
Description | g3p | ||||
Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O20] g3p_R | |||||
---|---|---|---|---|---|
Description | g3p | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O21] Ompa_linker_F | |||||
---|---|---|---|---|---|
Description | For standardisation of OmpA linker (BBaK103006) | ||||
Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O22] Ompa_linker_R | |||||
---|---|---|---|---|---|
Description | For standardisation of OmpA linker (BBaK103006) | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O23] OmpAsignal-TE3_seq_Fw | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | CACAAATAGCGAAAGATGAC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O24] OmpAsignal-TE3_seq_Rw | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | ATCATCTGCGGGTAATGATG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O25] VAMP2_Seq_Fw | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | ACTCACTATAGGGAGACCACA | ||||
Designer | G. Cambray | ||||
Not Tested |
[O26] VAMP2_Seq_Rv | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | GGGCTTTGTTAGCAGCCGGAT | ||||
Designer | G. Cambray | ||||
Not Tested |
[O27] ClyA_seq_Fw | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | GCAGCTATTTCCAGTCACAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O28] ClyA_seq_Rw | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | CGCCCGCAGCAATAGAATAG | ||||
Designer | G. Cambray | ||||
Not Tested |
[O29] PFecA_Fw | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC | ||||
Designer | G. Cambray | ||||
Not Tested |
[O30] PFecA_Rv | |||||
---|---|---|---|---|---|
Description | ??? | ||||
Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT | ||||
Designer | G. Cambray | ||||
Not Tested |
[O31] clyA_RBS_Fw | |||||
---|---|---|---|---|---|
Description | amplify clyA with RBS in the primer (std 10) | ||||
Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC | ||||
Designer | G. Cambray | ||||
Not Tested |