Team:Paris/Freezer Primers

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Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


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Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Primers

[O1] Rev P1
Description tolR
Sequence (5' > 3') TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG
Designer D. Bikard
Worked


[O2] Fwd P2
Description tolR (DO NOT USE ERROR)
Sequence (5' > 3') TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG
Designer D. Bikard
Worked


[O3] Rev P3
Description tolA
Sequence (5' > 3') TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG
Designer D. Bikard
Worked


[O4] Rev P4
Description tolA (ERROR: Not designed for N-term fusion)
Sequence (5' > 3') TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC
Designer D. Bikard
Worked


[O5] Rev P5
Description tatABC
Sequence (5' > 3') TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC
Designer D. Bikard
Worked


[06] Fwd P6
Description tatABC (without RBS)
Sequence (5' > 3') TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG
Designer D. Bikard
Worked


[O7] Rev P7
Description clyA fusion
Sequence (5' > 3') TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC
Designer D. Bikard
Worked


[O8] Fwd P8
Description clyA fusion (ERROR: No poly-Gly linker)
Sequence (5' > 3') TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA
Designer D. Bikard
Worked


[O9] clyA_Rw
Description clyA
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG
Designer G. Cambray
Not Tested


[O10] clyA_fusion_polyG_Rw
Description clyA fusion (with 5-Gly linker)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG
Designer G. Cambray
Not Tested


[O11] ompA_signal_Fw
Description ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG
Designer G. Cambray
Not Tested


[O12] ompA_signal_fusion_Rw
Description ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC
Designer G. Cambray
Not Tested


[O13] tolR_II_Fusion_Fw
Description For fusion of tolR domain II (standard 23)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC
Designer G. Cambray
Not Tested


[O14] tatABC_RBS_Fw
Description Amplification from tatA - contains RBS
Sequence (5' > 3') TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG
Designer G. Cambray
Not Tested


[O15] TE3_fusion_Fw
Description Fusion of N-term domain of colicin E3 (standard 23)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC
Designer G. Cambray
Not Tested


[O16] TE3_Rw
Description Amplification of N-term domain of colicin E3 (standard 23)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG
Designer G. Cambray
Not Tested


[O17] Tg3p_Rw
Description Amplification of N-term domain of M13's g3p
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC
Designer G. Cambray
Not Tested


[O18] Tg3p_fusion_Fw
Description Fusion of N-term domain of M13's g3p (standard 23)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG
Designer G. Cambray
Not Tested


[O19] g3p_F
Description g3p
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG
Designer G. Cambray
Not Tested


[O20] g3p_R
Description g3p
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG
Designer G. Cambray
Not Tested


[O21] Ompa_linker_F
Description For standardisation of OmpA linker (BBaK103006)
Sequence (5' > 3') TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG
Designer G. Cambray
Not Tested


[O22] Ompa_linker_R
Description For standardisation of OmpA linker (BBaK103006)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC
Designer G. Cambray
Not Tested


[O23] OmpAsignal-TE3_seq_Fw
Description  ???
Sequence (5' > 3') CACAAATAGCGAAAGATGAC
Designer G. Cambray
Not Tested


[O24] OmpAsignal-TE3_seq_Rw
Description  ???
Sequence (5' > 3') ATCATCTGCGGGTAATGATG
Designer G. Cambray
Not Tested


[O25] VAMP2_Seq_Fw
Description  ???
Sequence (5' > 3') ACTCACTATAGGGAGACCACA
Designer G. Cambray
Not Tested


[O26] VAMP2_Seq_Rv
Description  ???
Sequence (5' > 3') GGGCTTTGTTAGCAGCCGGAT
Designer G. Cambray
Not Tested


[O27] ClyA_seq_Fw
Description  ???
Sequence (5' > 3') GCAGCTATTTCCAGTCACAG
Designer G. Cambray
Not Tested


[O28] ClyA_seq_Rw
Description  ???
Sequence (5' > 3') CGCCCGCAGCAATAGAATAG
Designer G. Cambray
Not Tested


[O29] PFecA_Fw
Description  ???
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC
Designer G. Cambray
Not Tested


[O30] PFecA_Rv
Description  ???
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT
Designer G. Cambray
Not Tested


[O31] clyA_RBS_Fw
Description amplify clyA with RBS in the primer (std 10)
Sequence (5' > 3') TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC
Designer G. Cambray
Not Tested

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Primers"