Team:TUDelft/20 August 2009

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Revision as of 21:42, 20 August 2009 by Tim Weenink (Talk | contribs)

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20 August 2009

Tim Weenink

We got colonies on yesterdays transformations. RbCl plates had about 20 colonies each, while Electrocompetent cells were hundreds of colonies. Finally an oldfashioned highly efficient transformation. I suspect transformations with larger parts (>1kb) work better.

I inoculated some of the colonies. These are the names of the samples:

R/E stands for RbCl/Electrocompetent (resp) 1C3 stands for the used backbone (pSB1C3) α ß stands for plate numbers 1,2 stands for colony numbers

  • !SIIR1C3α1
  • !SIIR1C3α2
  • !SIIR1C3ß1
  • !SIIR1C3ß2
  • !SIIE1C3α1
  • !SIIE1C3α2
  • !SIIE1C3ß1
  • !SIIE1C3ß2


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