Team:TUDelft/9 September 2009

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9 September 2009

Sriram

Today I did the miniprep of the cultures inoculated yesterday. Also did a restriction to analyse the sizes of the products. It can be seen in the gel image below.

Sriram-Delay090909new1.jpg

From the gel it is clear that we have all the assemblies of proper sizes. The only troubling thing was the positive control GFP glowing colony gave a correct band while the non-GFP glowing colony is not of proper size. It is possible due to different assemblies that could be got from electroporation of ligated products.