Only a few colonies were found this morning =(. Unfortunately the number of colonies wasn´t enough to compare and decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.
New biobricks
Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).
We transformed the thermocompetent E. coli (Protocol 3) with the ligations and plated in LB+Amp media.
Raíssa and Taís
ColiGuard
Miniprep Results
We ran an agarose gel with the minipreps samples (proccedure performed yesterday).
According to both pictures, we sucessfully recovered all biobricks! :)
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