Team:Paris/Production overview

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Overview

• Introduction
• A. Tol/pal
• B. Our strategy
• C. Construction

Introduction

Several bacteria, including E. coli, have been shown to produce vesicles for various reasons (METTRE UNE REF ET CITER QUELQUES RAISONS). In our project, we want to optimize vesicle production to develop a long distance communication system between gram-negative bacteria. Outer membrane vesicles (OMVs) production can be enhanced through the destabilization of the outer membrane. We found in the literature that the Tol/Pal system could be a good target for this purpose^[1]. The Tol/Pal system of Escherichia Coli is indeed required to maintain the outer membrane integrity. It is composed of five membrane proteins, TolA, TolB, TolQ, TolR and Pal, included in two complexes..^[2][3]

See Tol/Pal part for more information and why we choose this system. Strategy and Construction part explains our aim in this OMV production system.

References

1. ^Transmembrane a-helix interactions are required for the functional assembly of the escherichia coli Tol complex. Lazzaroni & Geli 1995 - [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179564 179564]
2. ^The Tol-Pal proteins of the Escherichia coli cell envelope an energized system required for outer membrane integrity. Lloubès & Journet 2001 - [http://www.ncbi.nlm.nih.gov/pubmed/11501670 11501670]
3. ^Improved methods for producing outer membrane vesicles in gram-negative bacteria. Henry & Lloubès 2004 - [http://www.ncbi.nlm.nih.gov/pubmed/15249060 15249060]