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Team:Paris/Production overview
From 2009.igem.org
iGEM > Paris > Vesicle production system > Overview
Introduction
Several gram-negative bacteria - including E. coli - have been shown to produce vesicles for various hypothetical reasons[1][2] (stress response, host-pathogen interaction...). In our project, we want to optimize vesicle production to develop a long distance communication system between gram-negative bacteria. Outer membrane vesicles (OMVs) production can be enhanced through the destabilization of the outer-membrane. We found in the literature that the Tol/Pal system could be a good target for this purpose[3]. The Tol/Pal system of Escherichia Coli is involved in anchoring the outer- to the inner-membrane and to the peptidoglycan layer. It is thus essential in maintaining membrane integrity. The system is composed of five membrane proteins (TolA, TolB, TolQ, TolR and Pal) associating in two complexes.[4][5]
See Tol/Pal part for more information and why we choose this system. Strategy and Construction part explains our aim in this OMV production system.
References
- ^Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response. McBroom AJ & Kuehn MJ 2007 - [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=17163978 17163978]
- ^Biogenesis of bacterial membrane vesicles. Deatherage BL & Cookson BT 2009 - [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=19432795 19432795]
- ^Transmembrane a-helix interactions are required for the functional assembly of the escherichia coli Tol complex. Lazzaroni & Geli 1995 - [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179564 179564]
- ^The Tol-Pal proteins of the Escherichia coli cell envelope an energized system required for outer membrane integrity. Lloubès & Journet 2001 - [http://www.ncbi.nlm.nih.gov/pubmed/11501670 11501670]
- ^Improved methods for producing outer membrane vesicles in gram-negative bacteria. Henry & Lloubès 2004 - [http://www.ncbi.nlm.nih.gov/pubmed/15249060 15249060]
