Team:Paris/Brainstorm

Protocols Freezer NoteBook Bibliography Parts WetLab DryLab

Team Collaborations Contacts Acknowledgements Links

Brainstorming

In this section, every single thought and train of thoughts will be unveiled !

We offer you the opportunity to find the answer you are looking for, or to discover new questions and reflexions. In order to satisfy your demand we choose to proceed by ideas. Furthermore you will follow the team step by step in his reflexion, we would also be very grateful if you wanted to share some thoughts with us.

• Internal meeting
• External meeting and participation to scientific non-iGEM linked events
• Brainstorming Slides

A. Internal meeting

We start the weekly meetings at the end of April, we quickly agreed to meet once a week, and Thursday seems to match with our respective timetables. Since we have found our subject of study, we can separate those before and those after the choice.

A.1. Before

at the very begin was chaos ! follow the descent to hell :

And the light embraced our universe ! In other word, an abundance of project. Some of them are not listed below otherwise it would have been a disgrace to this competition.

chronological order:

• eucaryotic plasmid
• multigenomic microorganism
• bactowifi
• bacterioclock: how to know what time is it on a petri dishes
• bactocompass
• bactoTV
• bacteriopatch : patch to monitorize disease

A.2. The transition

The iGEM WEEKEND !!!!!!

and the application for the project that followed :

• bactotransistor
• The TCP/Bacterial Protocol.
• bact-arkanoïd.
• christmas garland.
• bactocolored vegetal.

A.3. After

At last we won ! the right to start thinking how, when and where to materialize our project. Infact we separate the idea of the application and the realization so that the steps could be standardize and reuse for other purposes .

We start focusing on 4 points :

1. production (of OMVs) :
   • pTol/pal
   • g3p
2. adressing (proteins in OMVs) :
   • TAT
   • Sec
3. fusion (of vesicles with the receiving bacteria):
   • Snare
   • adhesines
   • g3p
   • natural fusion
4. transduction (of the signal):
   • ABC transporter
   • two-component system

and then We ...... wait for the rest to come !

B. External meeting and participation to scientific non-iGEM linked events

Some of us have assisted to specific conference.

• 05/04/2009: "From Synthetic Chemistry to Synthetic Biology" , amphithéâtre Maurice Halbwachs, Collège de France

• 05/28/2009: "Will we be able to create a synthetic bacterium ?" with Antoine Danchin Professor from the Pasteur institute SYLVAIN

• 06/04/2009: "Engineering the living 2.0 , the synthetic biology in questions." Centre Pompidou [http://www.vivagora.org find here the conference and debate to come]

• 06/09/2009: "Learning from bacteria about information processing" Prof E. Ben -Jacob at the CRI SYLVAIN

We have participated in a event that aims to popularize science for all kind of public.

• 07 / 22 & 23 & 24 / 2009 "Festival des sciences" organized by Paris montagne at the ENS ULM

C. Brainstorming Slides

Here 5 powerpoints used when the team was brainstorming on the project.

• p1 : Design bacteria which are able to answer to a signal by producing vesicles.
• p2 : Zoom on Outer Membrane Vesicles.
• p3 : To a synthetic membrane vesicle
• p4 : Pre-Existent BioBricks
• p5 : First proposition for our constructions