Team:PKU Beijing/Notebook/AND Gate 1/AraC SupD Assembly

2009.8.3

00:00
Ligation:
Insert: pcr1, pla1
Vector: SupD, 1-12O

12:00
Transformation: yesterday’s ligation product

21:45
Grow the strains of pla1+SupD1, pcr1+1-12O1~4, pla1+1-12O1~4

22:10
Ligation:
Insert: pcr1
Vector: SupD

2009.8.4

11:00
Transformation: pcr1+SupD

13:00
Mini-prep: pla+1-12O1~4, pcr+1-12O1~4, plas1

18:30
Double-enzyme digestion: pla+1-12O3, pcr+1-12O1, pla+SupD1

22:30
Double-enzyme digestion: pla+1-12O3, pcr+1-12O1

2009.8.5

11:00
Sent samples for sequencing

16:00
Ligation:
Insert: pcr1, pla2
Vector: 1-12O

2009.8.6

15:00
Inducing test
Strain: pla+1-12O1, pcr+1-12O1

15:30
Double-enzyme digestion
Plasmid: pla+1-12O2, pcr+1-12O2

2009.8.7

15:00
Double-enzyme digestion: pcr1~6