User contributions
From 2009.igem.org
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- 01:49, 21 October 2009 (diff | hist) Team:Brown/Project
- 01:47, 21 October 2009 (diff | hist) Team:Brown/Project (→Project Abstract)
- 01:45, 21 October 2009 (diff | hist) Team:Brown/Project S.epidermidis
- 01:45, 21 October 2009 (diff | hist) Team:Brown/Project S.epidermidis
- 01:44, 21 October 2009 (diff | hist) Team:Brown/Project S.epidermidis
- 01:43, 21 October 2009 (diff | hist) Team:Brown/Team
- 01:41, 21 October 2009 (diff | hist) Team:Brown/Team
- 01:38, 21 October 2009 (diff | hist) Team:Brown/Team
- 21:39, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/CFU (New page: {{Brown}} How to calculate CFU’s ---- (pg plasmid DNA/total reaction volume) x plated volume = pg DNA plated (avg # of colonies/pg DNA plated) x (10^6 pg / µg) = (# of transf...)
- 21:36, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/immunoblotting (New page: {{Brown}} Immunoblotting (Western Blotting) Adapted for Spot-Blotting ---- Reagents needed: 1) Blotto (per liter, 10.8 gms NaCl; 20 mM Trip, pH 8.0; 30 gms not-fat dry milk; 0.0...)
- 21:35, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/redigest (New page: {{Brown}} DNA digestion protocol & hints ---- Overview: Although it is pretty standard to digest DNA with restriction enzymes, here are a standardized protocol and some hints...)
- 21:34, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/glycerolstocks (New page: {{Brown}} Glycerol Stocks ---- Every construct, whether created in the Stockinger lab or imported from another lab into the Stockinger lab, must be put away and maintained as a ...)
- 21:31, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/PCR
- 21:30, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/PCR (New page: {{Brown}} Protocol: Polymerase Chain Reaction ---- Background/Purpose Polymerase Chain Reaction (PCR) is used to amplify DNA segments by way of template strands, primers, and DNA poly...)
- 21:29, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/primerresusp (New page: {{Brown}} Primer resuspension ---- Goal: 5pM/ul. This is a 1xstock solution. Protocol: 1) Identify the nM amount on the tube and resuspend the primers in 10x (in ul) water, ...)
- 21:28, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/movingdna
- 21:28, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/movingdna (New page: {{Brown}} Moving DNA (subcloning) Often pieces of DNA (biobricks, PCR fragments, promoters, etc) need to be moved into a different vector, combined with other pieces, or modified ...)
- 21:27, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:26, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:26, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics (→Bacterial Basics)
- 21:26, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:25, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:25, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:25, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:25, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols/bacterialbasics
- 21:24, 20 October 2009 (diff | hist) N Team:Brown/Notebook Protocols/bacterialbasics (New page: {{Brown}} Bacterial Basics ---- Bacteria Structure ---- Bacteria are prokaryotic organisms. They are surrounded by a firm cell wall that helps maintain their structure. Bacteria h...)
- 21:21, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols
- 21:20, 20 October 2009 (diff | hist) Team:Brown/Notebook Protocols
- 21:09, 20 October 2009 (diff | hist) Team:Brown/Notebook Meetings/9-11-09
- 21:07, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/9-11-09 (New page: {{Brown}} 9/11/09 iGEM Meeting SFH 218 8:00 AM: Gary reminds us of our responsibility. We need to plan this semester! These next few weeks are the most important Adrian: Deadlines ...)
- 21:05, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 21:03, 20 October 2009 (diff | hist) Team:Brown/Notebook Meetings/6-8-09
- 21:03, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/6-8-09 (New page: {{Brown}} iGEM Meeting June 8, 2009 6:00 PM PST 6:19: We discovered we had video chat capabilities. It made the meeting AWESOME. 6:23: Possible 3rd kill switch: SulA stops FtsZ gene...)
- 21:02, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 21:02, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 21:00, 20 October 2009 (diff | hist) Team:Brown/Notebook Meetings/6-2-09 (top)
- 21:00, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/6-2-09 (New page: {{Brown}} Brown iGEM Meeting June 3, 2009 WebEx Conference Call Attendees: Steph, Will, Indu, Eli, Michael 8:20: Steph will be researching S. Aureus expression vectors, and the natur...)
- 20:59, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 20:59, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/5-31-09 (New page: {{Brown}} Brown iGEM 2009 WebEx Conference Call May 31, 2009 8:00 PM Attendees: Will, Eli, Indu, Michael 8:00: Will introduces new idea to deal with localization problem using S. ...)
- 20:57, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 18:14, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/5-27-09 (New page: {{Brown}} Brown iGEM 2009 Phone Conference 5/27/09 (Pacific Time) 8:20PM: Meeting starts after a lot of technical difficulties 8:22: Brad Lowell at Harvard does not have UCP2, Inv...)
- 18:12, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 18:11, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/5-6-09 (New page: {{Brown}} iGEM 2009 Meeting May 6, 2009 5:09: Meeting begins in SFH 218, with Adrian and Dana. Today we are presenting our powerpoints for Friday’s panel meeting 5:09: Minoo: eng...)
- 18:11, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 18:11, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 18:10, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/5-4-09 (New page: {{Brown}} iGEM 2009 Meeting May 4, 2009 6:13PM: Meeting starts in SFH Auditorium. Discussed War War Ko’s name. 6:14: Project ideas. Adding Minoo’s “Pro Bread” to the list ...)
- 18:10, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 18:09, 20 October 2009 (diff | hist) N Team:Brown/Notebook Meetings/4-22-09 (New page: {{Brown}} iGEM 2009 Meeting April 22, 2009 9:40PM: Meeting begins in the SciLi Basement 9:43: iGEM account, Google groups account, Mathworks; email Neil for google group invitation!...)
- 18:09, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
- 18:08, 20 October 2009 (diff | hist) Team:Brown/Notebook meetings
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