construction result
Input promoters construction:
In the AND gate, we used five different inducible promoters as inputs: pBad (respond to L-arabinose), pSal (respond to salicylate), pLac (respond to IPTG), pTet (respond to tetracycline), luxP (respond to homoserine lactone). When the inducing small molecule in presence, the promoter is activate, for example, IPTG can activate pLac.
We have constructed four of these inducible systems (including the activator or repressor protein and promoter) and used one directly from other parts. The general pattern is like this:
They are:
BBa_K228004: NahR-pSal system;
BBa_K228009: AraC-pBad system;
BBa_K228817: lacI-pLac system;
BBa_K228818: tetR-pTet system.
They are not only composite parts which can be used in our project, but also valuable devices employed in other designs or circuits.
For the luxR-luxP system, we simply utilized the parts: BBa_J09855, which is designed and constructed by iGEM2006_Pennstate.
Input promoters test:
In order to characterize the AND gate (e.g. the transfer function), the activities of these promoters should be measured independently by constructing GFP (with strong RBS B0034) downstream the promoters and testing the output in response to the small-molecular inducers.
aTc Sensor
IPTG Sensor
HSL Sensor
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