"
Team:TUDelft/27 August 2009
From 2009.igem.org
Lab Notebook
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
27 August 2009
Saeed
The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.
| lane | Part | Expected Plasmid Size | Status |
| 1 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | ||
| 2 | !Za | 1934 | no band |
| 3 | !Zb | 1934 | ok |
| 4 | !Zg | 1934 | ok |
| 5 | !Zh | 1934 | ok |
| 6 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] |
!Zb and !Zg seems to be ok. !Zh looks a bit bigger, but this also could be because of the gel. !Zb or !Zg should be used for sequencing.
Daniel
Miniprep of locks and keys in biobrick´s plasmid backbones and some previous assemblies
| Part | [DNA] ng/uL |
| LMA | 46.1 |
| KMA | 58.8 |
| LWA | 59.1 |
| KWA | 54.7 |
| 2B | 64.4 |
| 1A | 122.2 |
| 1B | 113.3 |
Restriction of Locks and keys to use them for assembly. The gel shows weird results.
Ligation of 1C, 2C, 3C and 4C
