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Team:TUDelft/30 July 2009
Lab Notebook
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30 July 2009
Tim Weenink
prepared assembly mixes for !B, !C and !D constructs. Standard Ginko assembly kit was used. DNA concentrations as below (these mixtures were added to 7.5 µl of digestion mixture):
| Assembly name | position | Components | volume in µl |
| !B | Upstream | !A DNA | 33.0 |
| H20 | 9.5 | ||
| Downstream | BBa_B0015 DNA | 10.0 | |
| H20 | 32.5 | ||
| Backbone | pSB1AC3 DNA | 6.5 | |
| H20 | 36 | ||
| !C | Upstream | BBa_R0040 DNA | 10.0 |
| H20 | 32.5 | ||
| Downstream | *I6 DNA | 5.5 | |
| H20 | 37.0 | ||
| Backbone | pSB1AC3 DNA | 6.5 | |
| H20 | 36 | ||
| !D | Upstream | *I6 DNA | 5.5 |
| H20 | 37.0 | ||
| Downstream | BBa_K145201 DNA | 18.0 | |
| H20 | 24.5 | ||
| Backbone | pSB1AC3 DNA | 6.5 | |
| H20 | 36 |
15:20 Plated assemblies
15:40 inoculated (2*4) *S and *T cultures
16:55 Inoculated 2 !A cultures
Calin
Plated three backbones left over from the transformation the other day. Did a digest on oriT-R with E and P.
Tim Vos
Minipreped pSB4C5, pSB.
