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Team:TUDelft/29 July 2009
From 2009.igem.org
Lab Notebook
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29 July 2009
Calin
Checked concentrations from yesterday's miniprep.
| Part | Concentration (ng/uL) |
| pSB1AK3 I | 92 |
| pSB1AK3 II | 77 |
| lambda_p GFP I | 119 |
| lambda_p GFP II | 108 |
| pLacI | 56.7 |
| RBS | 95.2 |
Todays gel:
| Well | Part | Expected Plasmid Size | Status |
| 1 | BBa_K142205 PCR | ||
| 2 | BBa_K142202 PCR | ||
| 3 | BBa_B0015 PCR | ||
| 4 | BBa_R0040 PCR | ||
| 5 | BBa_K145201 PCR | ||
| 6 | *I3 uncut | ||
| 7 | *I3 BglI cut | ||
| 8 | *I3 BglI+I-SceI cut | ||
| 9 | *I6 uncut | ||
| 10 | *I6 BglI cut | ||
| 11 | *I7 BglI+I-SceI cut | ||
| 12 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | ||
| 13 | pSB1AK3 I | 3864 | ✖ |
| 14 | pSB1AK3 II | 3864 | ✖ |
| 15 | λp-GFP I | 3011 | ✔ |
| 16 | λp-GFP II | 3011 | ✔ |
| 17 | pLacI | 2279 | ✔ |
| 18 | rbs | 2091 | ✔ |
Sriram
We planned to make electro competent cells today but we got the required OD of 0.5 to 1 a bit late. We hoped to make cells if the meeting finishes quicker, but sadly it took longer than expected hence we had to waste the culture that was grown. Laura gave a new protocol for preparing chemically competent cells and also 6 ml of buffer required. We decided to prepare the cells tomorrow. Hence again made some 3 culture tubes of top10 cells.
Orr
Got the source code in R for the modelling of conjugation, and already looked at how it works.
