Team:Paris/constructions

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Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


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Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Constructions

  • Plasmid A


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC ((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC (OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] OmpA linker standardisé ?? BBa_????
(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] autre possibilité (souf) ?? BBa_????
CC! ((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((rbs - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC ((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC ((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ??fusion N term de cly A (autre possibilité) BBa_????
CC! ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC! ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] 2*Pbad et 2*ter?? (souf) ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????



  • Plasmid B


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
GC ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 BBa_blabla :: BBa_tralala in truc Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) 12 BBa_pouet pouet
CC (Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_???? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CL (rbs - OmpAsignal :: TolR II) BBa_???? oligo designed, ?? BBa_????
CC (Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) ?? BBa_????
CC (Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????


  • Plasmid C


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? ?? BBa_????


CC PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] BBa_??? ?? BBa_????