Team:TUDelft/14 August 2009
Lab Notebook
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14 August 2009
Tim Weenink
First did ligation of !E and !F with the parts that were not excised from gel.
Component for !E | volume (uL) |
*S PCR (upstream) | 2 |
!C PCR (downstream) | 2 |
pSB1AK3 from !A assembly | 2 |
T4 ligase buffer | 2 |
T4 ligase | 1 |
H2O | 11 |
Component for !F | volume (uL) |
!B PCR (upstream) | 2 |
!D PCR (downstream) | 2 |
pSB1AK3 from !A assembly | 2 |
T4 ligase buffer | 2 |
T4 ligase | 1 |
H2O | 11 |