Team:TUDelft/18 August 2009

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Lab Notebook

July
MTWTFSS
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August
MTWTFSS
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31
September
MTWTFSS
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October
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18 August 2009

Calin

Yesterdays 20 well 1% gel:


Well Part Expected Plasmid Size Status
1
2
3
4
5
6 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
7 Colony PCR 1A - R751ΔtrbK 1616
8 Colony PCR 1A - R751ΔtrbK 1616
9 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348
10 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
11 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔

Yesterdays 26 well 2% gel:


Well Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
21
22 Colony PCR 1A - R751ΔtrbK 1616
23 Colony PCR 1A - R751ΔtrbK 1616
24 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348
25 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
26 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398

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