Lab Notebook
24 August 2009
Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies
Daniel
Digestion of assemblies in order to do the second round of assembly.
Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR
Right Gel (2%)
Well | Assembly | Expected Plasmid Size | Status
|
1 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | | ✔
|
2 | 4A | 824 | ?
|
3 | 5A | 1040 | ✔
|
4 | 2A | 214 | ?
|
5 | 3A | 183 | ?
|
6 | 1A | 1076 | ?
|
7 | PCR 1 | |
|
8 | PCR 2 | |
|
9 | PCR 3 | |
|
10 | PCR 4 | |
|
11 | PCR 5 | |
|
12 | PCR 6 | |
|