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Team:TUDelft/31 August 2009
Lab Notebook
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31 August 2009
Daniel
I have some doubts about the PCR results of the locks and keys so I decided to do a second round. However, I continued the transformation of 1-4 C assemblies.
PCR purification gel (2%):
| Well | Part | Expected Size | Status |
| 1 | |||
| 2 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | ||
| 3 | Lock Medium RBS | 90 | ? |
| 4 | Key Medium RBS | 127 | ? |
| 5 | Lock Weak RBS | 92 | ? |
| 6 | Key Weak RBS | 129 | ? |
| 7 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] |
I did transformation and culture in plates and tubes of 1C-4C, 1A, 1-3B and the biobricks J23119 and S03520. These last biobricks are a constitutive promoter and a LacI generator because Tim found that top 10 cells don't produce LacI
