Lab Notebook
4 September 2009
Tim Weenink
I transformed all four batches of competent cells with !SIII ligation mix. Plates (Cam) were left on the bench in a plastic bag over the weekend. All the time constants in the electroporation were 4.4 or 4.5 (which is good). Also two plates without selection marker were streaked to check if the competent cells were viable at all.
Saeed
I made minipreps form 6 overnight cultures of !P in order to check the assembly of !P. These are called !P1 to !P6.
| Part | [DNA] ng/uL
|
| !P1 | 1001.1
|
| !P2 | 92.3
|
| !P3 | 98.0
|
| !P4 | 102.2
|
| !P5 | 89.1
|
| !P6 | 86.1
|
The isolated plasmide were restricted with E and P in order to check fragment size. !P1, !P3 and !P5 are also cut upstream.
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