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Team:TUDelft/8 September 2009
Lab Notebook
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8 September 2009
Tim Weenink
Today: Plasmid isolation: out of 10 !SIII inoculated cultures, only 3,5 and 6 showed proper growth. The others showed very low growth and will be incubated for more time. Growth/low growth is not related to colony size, which seemed to have two growing modes: big colonies and small colonies.
Did miniprep and made glycerol stock and did E-P restriction of the following:
| part | concentration | 260/280 | 260/230 |
| !SIII3 | 100.1 | 2.00 | 2.27 |
| !SIII4 | 3.3 | 2.94 | 2.94 |
| !SIII5 | 102.4 | 1.93 | 2.28 |
| !SIII6 | 117.2 | 1.96 | 2.24 |
Also did E-P restriction of the pSB4C5 backbone. This was all gelled:
Finally I did a ligation of pLacI (upstream), *T1 (BBa_K142202: I-SceI homing endonuclease with RBS & double terminator) (downstream) and pSB4C5 as a backbone.
