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Team:TUDelft/11 September 2009
Lab Notebook
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11 September 2009
Tim Weenink
Ligations:
upstream: !SIII5
downstream: !Zg
Backbone: pSB4A5 (tube III)
Upstream: !SIII5
Downstream: !Zg
Backbone: pSB1A3
Analysed rest of !SIII samples on nanodrop:
!SIII1: 30.8 ng/ul
!SIII2: 79.5 ng/ul
!SIII8: 55.8 ng/ul
!SIII9: 103.8 ng/ul
!SIII10: 32.5 ng/ul
Did SDS page gel of IPTG induced !SIII3 and !SIII5 strains and non induced controls. !SIII3 showed an extra band between 20 and 30 kDa, which is roughly the expected band length. The lanes of !SIII5 were not very well visible.
Did transformation with the above ligations. These are constructs !G and !H.
