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Team:Newcastle/Labwork/24 September 2009
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===Introduction=== | ===Introduction=== | ||
| - | + | * We prepared kinA and pGFP-rrnB digested fragments yesterday, we'll carry on the next step as gel extraction and ligation and transformation. | |
| - | + | * For the double clone part, we need to ligate it to pMutin4 vector. | |
| - | + | ||
<br> | <br> | ||
===Experiment procedure=== | ===Experiment procedure=== | ||
| - | ==== | + | ==== Gel extraction ==== |
| - | ==== | + | * Follow the standard procedure of Gel Extraction Kit(Sigma). |
| - | + | ==== Ligation ==== | |
| + | * Ligate kinA to pGFP-rrnB | ||
| + | * Ligate digested PCR clean-up psc fragment to pMutin4 | ||
| + | ** this psc fragment refer to the cwlJ:sleB fragment with HindIII and BamHI restriction sites at the end of each site. | ||
==== 3 ==== | ==== 3 ==== | ||
Revision as of 23:31, 20 October 2009
Project
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Wet Lab
Meetings
Planning
Links
Formal Lab Session - 24th September 2009
Stochastic switch team
Today we did minipreps of the ara/sspb/pSB1AT3 cultures which were then digested with EcoRI and SpeI. These were run on an 0.8% gel. We expected to see a ~600bp band as well as the plasmid backbone and the gel photograph seemed fairly convincing:
We set up a 50ml midiprep culture from the culture corresponding to lane 9 which seemed to have the clearest band.
Chassis team
Introduction
- We prepared kinA and pGFP-rrnB digested fragments yesterday, we'll carry on the next step as gel extraction and ligation and transformation.
- For the double clone part, we need to ligate it to pMutin4 vector.
Experiment procedure
Gel extraction
- Follow the standard procedure of Gel Extraction Kit(Sigma).
Ligation
- Ligate kinA to pGFP-rrnB
- Ligate digested PCR clean-up psc fragment to pMutin4
- this psc fragment refer to the cwlJ:sleB fragment with HindIII and BamHI restriction sites at the end of each site.
3
Conclusion
[[Image:|400px|center]]
Futher plan
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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