Team:PKU Beijing/Project/AND Gate 1/Design

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Revision as of 10:05, 10 October 2009

Project

AND Gate 1 Design

Mechanism

Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1].


fig1. The mechanism of the AND Gate
J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007)
Environmental signal integration by a modular AND gate Molecular Systems Biology 3:133

Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter.

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   [[Image:PKU_AND_Gate_Mechanism.png]]
   fig1. The mechanism of the AND Gate
-  J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007) ''Environmental signal integration by a modular AND gate'' '''Molecular Systems Biology 3:133'''
+  J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007)
+ ''Environmental signal integration by a modular AND gate'' '''Molecular Systems Biology 3:133'''
 Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with  amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter.
 {{PKU_Beijing/Foot}}