Team:PKU Beijing/Project/AND Gate 1/Design
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(New page: ==='''Mechanism'''=== Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1]. Image:PKU_AND_Gate_Mechanism.png fig...) |
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==='''Mechanism'''=== | ==='''Mechanism'''=== | ||
Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1]. | Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1]. | ||
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Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter. | Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter. | ||
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Revision as of 09:59, 10 October 2009
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