Team:PKU Beijing/Project/AND Gate 1/Design
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Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1]. | Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1]. | ||
- | [[Image:PKU_AND_Gate_Mechanism.png]] | + | [[Image:PKU_AND_Gate_Mechanism.png]] |
fig1. The mechanism of the AND Gate | fig1. The mechanism of the AND Gate | ||
Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter. | Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter. | ||
{{PKU_Beijing/Foot}} | {{PKU_Beijing/Foot}} |
Revision as of 10:01, 10 October 2009
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