Team:PKU Beijing/Project/AND Gate 1/Design
From 2009.igem.org
(Difference between revisions)
(→Mechanism) |
|||
Line 12: | Line 12: | ||
The AND Gate can be further decoupled into a input interface, a core, and a output interface. | The AND Gate can be further decoupled into a input interface, a core, and a output interface. | ||
[[Image:PKU_AND_Gate.png|500px|center|thumb|fig2. '''AND Gate Biological Components''']] | [[Image:PKU_AND_Gate.png|500px|center|thumb|fig2. '''AND Gate Biological Components''']] | ||
- | |||
The input interface includes two inducible promoter which can be induced to expression by two different environmental small molecules. Core of the AND Gate is the T7 polymerase coding sequence with amber mutations and supD tRNA. And the Output interface is actually a T7 promoter, which regulate the expression of the downstream component. | The input interface includes two inducible promoter which can be induced to expression by two different environmental small molecules. Core of the AND Gate is the T7 polymerase coding sequence with amber mutations and supD tRNA. And the Output interface is actually a T7 promoter, which regulate the expression of the downstream component. | ||
Line 25: | Line 24: | ||
There are two ways, actually, to put the generator upstream of the inducible promoter. | There are two ways, actually, to put the generator upstream of the inducible promoter. | ||
As shown in fig3. | As shown in fig3. | ||
- | + | [[Image:PKU_Generator_Promoter_Reverse.png|300px|left|thumb|fig3A. '''One Construct''']] | |
- | + | [[Image:PKU_Generator_Promoter_Forward.png|300px|right|thumb|fig3A. '''Another Construct''']] | |
- | + | ||
- | + | ||
1. The constitutive promoter is cloned back to back with the inducible promoter, thus transcription of the activator or the repressor is opposite of the inducible promoter. 2. The generator transcripts in the same direction of the inducible promoter, the constitutive promoter is prevented to interfere with the inducible promoter by the terminator. However, any terminator is leaky, the first way is the better way to prevent interference. | 1. The constitutive promoter is cloned back to back with the inducible promoter, thus transcription of the activator or the repressor is opposite of the inducible promoter. 2. The generator transcripts in the same direction of the inducible promoter, the constitutive promoter is prevented to interfere with the inducible promoter by the terminator. However, any terminator is leaky, the first way is the better way to prevent interference. | ||
Revision as of 11:38, 10 October 2009