Team:PKU Beijing/Project/AND Gate 1/Inducible System Result
From 2009.igem.org
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==='''Construction result'''=== | ==='''Construction result'''=== | ||
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Input promoters construction: | Input promoters construction: | ||
Before the construction of AND gates, five different inducible promoters were chosen as inputs: pBad (respond to L-arabinose), pSal (respond to salicylate), pLac (respond to IPTG), pTet (respond to tetracycline) and luxP (respond to homoserine lactone). When the inducing small molecules are in presence, the corresponding promoter is activated, for example, IPTG can induce pLac. | Before the construction of AND gates, five different inducible promoters were chosen as inputs: pBad (respond to L-arabinose), pSal (respond to salicylate), pLac (respond to IPTG), pTet (respond to tetracycline) and luxP (respond to homoserine lactone). When the inducing small molecules are in presence, the corresponding promoter is activated, for example, IPTG can induce pLac. | ||
We have constructed four of these inducible systems (including the activator or repressor protein and promoter), from basic parts, and used one directly from other composite parts. The general pattern is like this: | We have constructed four of these inducible systems (including the activator or repressor protein and promoter), from basic parts, and used one directly from other composite parts. The general pattern is like this: | ||
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They are: | They are: | ||
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They are not only composite parts which can be used in our project, but also valuable devices which can be employed in other designs or circuits. | They are not only composite parts which can be used in our project, but also valuable devices which can be employed in other designs or circuits. | ||
For the luxR-luxP system, we simply utilized the parts: BBa_J09855, which is designed and constructed by iGEM2006_Pennstate. | For the luxR-luxP system, we simply utilized the parts: BBa_J09855, which is designed and constructed by iGEM2006_Pennstate. | ||
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Input promoters test: | Input promoters test: | ||
In order to characterize the AND gate (e.g. the transfer function), the activities of these promoters should be measured independently by constructing GFP (with strong RBS B0034) downstream the promoters and testing the output in response to the small-molecular inducers. | In order to characterize the AND gate (e.g. the transfer function), the activities of these promoters should be measured independently by constructing GFP (with strong RBS B0034) downstream the promoters and testing the output in response to the small-molecular inducers. | ||
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Therefore we successfully fused these promoters to GFP and test results are following: | Therefore we successfully fused these promoters to GFP and test results are following: |
Revision as of 12:46, 20 October 2009
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