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Team:Paris/Freezer Digestion Products
From 2009.igem.org
(Difference between revisions)
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| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | PelB leader sequence |
| ??? | | ??? | ||
| ??? | | ??? | ||
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| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | Double terminator bidirectionnal (B0012-B0011) |
| ??? | | ??? | ||
| ??? | | ??? | ||
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| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | pLac |
| ??? | | ??? | ||
| ??? | | ??? | ||
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| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | TetR repressible promote |
| ??? | | ??? | ||
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
| - | |- style="background: # | + | |- style="background: #ccchttps://2009.igem.org/wiki/index.php?title=Team:Paris/Freezer_Digestion_Products&action=edit§ion=2cff; text-align: center;" |
|style="background:#FFFF00"| D5 | |style="background:#FFFF00"| D5 | ||
| P8 | | P8 | ||
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| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | pBad/araC |
| ??? | | ??? | ||
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Revision as of 14:49, 5 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enz. 1 | Enz. 2 | Size | Description | Product Left | []ng/µL | Date |
| D1 | P3 | BBa-J32015 | XbaI | PstI | Size | PelB leader sequence | ??? | ??? | 08/04 |
| D2 | P4 | BBa-B0014 | XbaI | PstI | Size | Double terminator bidirectionnal (B0012-B0011) | ??? | ??? | 08/04 |
| D3 | P5 | BBa-I14032 | XbaI | PstI | Size | pLac | ??? | ??? | 08/04 |
| D4 | P7 | Bba-R0040 | XbaI | PstI | Size | TetR repressible promote | ??? | ??? | 08/04 |
| D5 | P8 | BBa-I0500 | XbaI | PstI | Size | pBad/araC | ??? | ??? | 08/04 |
| D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | ??? | ??? | 08/04 |
| D7 | ??? | ??? | XbaI | PstI | Size | ??? | ??? | ??? | 08/04 |
