Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(Digestion Products)
(Digestion Products)
Line 42: Line 42:
|- style="background: #0d3e99; text-align: center; color:white;"
|- style="background: #0d3e99; text-align: center; color:white;"
|width=5%| Number
|width=5%| Number
-
|width=20%| Product Name
+
|width=20%| Name
-
|width=20%| Matrix Name
+
|width=20%| Description
 +
|width=20%| Matrix ID
|width=5%| Enz. 1
|width=5%| Enz. 1
|width=5%| Enz. 2
|width=5%| Enz. 2
-
|width=5%| Size
+
|width=5%| Size (bp)
-
|width=20%| Description
+
|width=10%| Digested?
-
|width=10%| Product Left
+
|width=10%| Purified?
-
|width=5%| []ng/µL
+
|width=5%| [ng/µL]
-
|width=5%| Date
+
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D1
|style="background:#FFFF00"| D1

Revision as of 12:57, 7 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7  ???  ??? XbaI PstI Size  ???  ???  ??? 08/04