Team:Paris/Freezer PCR products

From 2009.igem.org

(Difference between revisions)
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|width=5%| ?
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05]
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A2 <span/ id="A2">
|width=5%| A2 <span/ id="A2">
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|width=10%| ?
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|width=10%| TE3_fusion N term
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|width=10%| [O15] TE3_fusion_Fw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]  
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|width=10%| [O16] TE3_Rw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O16 O16 ]
|width=5%| ?
|width=5%| ?
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| 1036bp
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|width=10%| 1036
|width=25%| Amplification of N-term domain of colicin E3
|width=25%| Amplification of N-term domain of colicin E3
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A3 <span/ id="A3">
|width=5%| A3 <span/ id="A3">
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|width=10%| ?
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|width=10%| clyA_fusion C term
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|width=10%| [O31] clyA_RBS_Fw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O31 O31 ]  
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|width=10%| [O10] clyA_fusion_polyG_Rw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O10 O10 ]
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|width=5%| ?
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|width=5%| ?(55.5)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| 987bp
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|width=10%| 987
|width=25%| Amplification of clyA with RBS in the primer
|width=25%| Amplification of clyA with RBS in the primer
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A4 <span/ id="A4">
|width=5%| A4 <span/ id="A4">
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|width=10%| ?
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|width=10%| OmpAsignal_fusion C term
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|width=10%| [O11] ompA_signal_Fw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O11 O11 ]  
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|width=10%| [O12] ompA_signal_fusion_Rw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O12 O12 ]
|width=5%| ?
|width=5%| ?
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=5%| ?
|width=5%| ?
|width=5%| ? ng/µL
|width=5%| ? ng/µL
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A5 <span/ id="A5">
|width=5%| A5 <span/ id="A5">
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|width=10%| ?
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|width=10%| tolRII_fusion N term
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|width=10%| [O1] Rev P1
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O13 O13 ]  
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|width=10%| [O13] tolR_II_Fusion_Fw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O1 O1 ]
|width=5%| ?
|width=5%| ?
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=5%| ?
|width=5%| ?
|width=5%| ? ng/µL
|width=5%| ? ng/µL
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A6 <span/ id="A6">
|width=5%| A6 <span/ id="A6">
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|width=10%| ?
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|width=10%| tatABC_RBS
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|width=10%| [O14] tatABC_RBS_Fw
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O14 O14 ]  
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|width=10%| [O5] Rev P5
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]
|width=5%| ?
|width=5%| ?
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=5%| ?
|width=5%| ?
|width=5%| ? ng/µL
|width=5%| ? ng/µL
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A?
|width=5%| A?
|width=10%| g3p_std
|width=10%| g3p_std
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|width=10%| [O19] g3p_F
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 O19 ]  
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|width=10%| [O20] g3p_R
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O20 O20 ]
|width=5%| 78°C
|width=5%| 78°C
|width=10%| P12
|width=10%| P12
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|width=5%| A?
|width=5%| A?
|width=10%| OmpA_L_std
|width=10%| OmpA_L_std
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|width=10%| [O21] Ompa_linker_F
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O21 O21 ]  
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|width=10%| [O22] Ompa_linker_R
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O22 O22 ]
|width=5%| 80°C
|width=5%| 80°C
|width=10%| P6
|width=10%| P6

Revision as of 12:50, 9 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Ref Name Oligo Fw Oligo Rev Tm used(Tm cal.) (°C) Matrix Length (bp) Comments Quantity [ ] (ng/µL) Date
A1 Tg3p_fusion N term O18 O17  ? (63.5) F4 274 Amplification of N-term domain of M13's g3p  ?  ? 08/05
A2 TE3_fusion N term O15 O16  ? F4 1036 Amplification of N-term domain of colicin E3  ?  ? 08/05
A3 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ? 08/05
A4 OmpAsignal_fusion C term O11 O12  ? F4 135bp Amplification of OmpA signal for export to periplasm  ?  ? ng/µL 08/05
A5 tolRII_fusion N term O13 O1  ? F4 279bp Amplification of tolR  ?  ? ng/µL 08/05
A6 tatABC_RBS O14 O15  ? F4 1634bp Amplification of tatA with RBS  ?  ? ng/µL 08/05
A? g3p_std O19 O20 78°C P12 1287bp For standardisation of g3p and adding SacI site  ?  ? ng/µL  ?
A? OmpA_L_std O21 O22 80°C P6 525bp For standardisation of OmpA linker (BBaK103006)  ?  ? ng/µL  ?



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_PCR_products"