Team:Paris/Freezer Strains (Glycerol stock)

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom | Freezer Strains (Glycerol stock)]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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==Freezer==
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<center>
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== Strains (Glycerol stock) ==
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[[Team:Paris/Freezer_Primers#Freezer | Primers]] -
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<html>
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[[Team:Paris/Freezer_PCR_products#Freezer | PCR products]] - 
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<style type="text/css">
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[[Team:Paris/Freezer_Plasmids#Freezer | Plasmids]] -
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#left-side {
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[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -
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    position: absolute;
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[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -
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    height: 23px;
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[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]]
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    width: 30px;
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}
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    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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</style>
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<div id="left-side"></div>
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<div id="middle-side"><center>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
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</center>
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<div id="right-side"></div>
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{|
{|
|- style="background: #c5c5c5;"
|- style="background: #c5c5c5;"
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|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
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<div id="paris_content_boxtop">
 
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== Strains (Glycerol stock) ==
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{|
{|
|- style="background: #c5c5c5;"
|- style="background: #c5c5c5;"
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|<strike>DH5&alpha;</strike>
|<strike>DH5&alpha;</strike>
|<strike>DH5&alpha; E. Coli strain for chimically competent</strike>
|<strike>DH5&alpha; E. Coli strain for chimically competent</strike>
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|[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
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|[https://2009.igem.org/Team:Paris/20_July_2009#Lab_work 07/20]
|- style="background: #cbcee0; text-align: center;"
|- style="background: #cbcee0; text-align: center;"
|S2<span/ id="S2">
|S2<span/ id="S2">
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|K12 MG 1655 &Delta;recA::Kan
|K12 MG 1655 &Delta;recA::Kan
|Standard E.Coli strain, Kan resistance
|Standard E.Coli strain, Kan resistance
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|[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
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|[https://2009.igem.org/Team:Paris/20_July_2009#Lab_work 07/20]
|- style="background: #cbcee0; text-align: center;"
|- style="background: #cbcee0; text-align: center;"
|S3 <span/ id="S3">
|S3 <span/ id="S3">
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|- style="background: #cbcee0; text-align: center;"
|- style="background: #cbcee0; text-align: center;"
|S13 <span/ id="S13">
|S13 <span/ id="S13">
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|style="background:#006400;"|K
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|style="background:#3333FF;"|A
|DH5&alpha;(BBa_K103006)
|DH5&alpha;(BBa_K103006)
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|DH5&alpha; E. Coli strain with BBa_K103006 in plasmid, Kan resistance
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|DH5&alpha; E. Coli strain with BBa_K103006 in plasmid, Amp resistance
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #cbcee0; text-align: center;"
|- style="background: #cbcee0; text-align: center;"
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|Ming Strain : RpoH-RFP-iBpA, RpoS-YFP-ying, RpoD-CFP-pZ
|Ming Strain : RpoH-RFP-iBpA, RpoS-YFP-ying, RpoD-CFP-pZ
|[https://2009.igem.org/Team:Paris/9_August_2009 08/09]
|[https://2009.igem.org/Team:Paris/9_August_2009 08/09]
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|- style="background: #cbcee0; text-align: center;"
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|S33 <span/ id="S33">
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|style="background:#3333FF;"|A
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|DH5&alpha;(BBa_K103006)
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|DH5&alpha; E. Coli strain with BBa_K103006 in plasmid, Amp resistance
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|[https://2009.igem.org/Team:Paris/14_August_2009 08/14]
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|- style="background: #cbcee0; text-align: center;"
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|S34 <span/ id="S34">
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|style="background:#006400;"|K
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|DH5&alpha;(pSB2K3)
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|DH5&alpha; E. Coli strain with pSB2K3 plasmid, Kan resistance
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|[https://2009.igem.org/Team:Paris/14_August_2009 08/14]
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|- style="background: #cbcee0; text-align: center;"
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|S35 <span/ id="S35">
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|
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|AA93
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|&Delta;fecA
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|[https://2009.igem.org/Team:Paris/15_August_2009 08/15]
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|- style="background: #cbcee0; text-align: center;"
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|S36 <span/ id="S36">
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|style="background:#FF0066;"|C
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|FecIR mutant
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|FecR constitutif
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|[https://2009.igem.org/Team:Paris/15_August_2009 08/15]
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|- style="background: #cbcee0; text-align: center;"
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|S37 <span/ id="S37">
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|style="background:#FF0066;"|C
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|FecIR wt
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|...
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|[https://2009.igem.org/Team:Paris/15_August_2009 08/15]
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|- style="background: #cbcee0; text-align: center;"
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|S38 <span/ id="S38">
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|style="background:#3333FF;"|A
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|FecA lacZ
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|...
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|[https://2009.igem.org/Team:Paris/15_August_2009 08/15]
|}
|}

Latest revision as of 15:25, 19 October 2009

iGEM > Paris > Freezer > Freezer Strains (Glycerol stock)


Strains (Glycerol stock)

Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol


Strains glycerol stock
Number R Name Description Date
S1 DH5α DH5α E. Coli strain for chimically competent 07/20
S2 K K12 MG 1655 ΔrecA::Kan Standard E.Coli strain, Kan resistance 07/20
S3 K W3110 Δnlp1 From Daniel Rodriguez, Kan resistance 07/23
TOP10 TOP10 Competent bacteria 07/27
S4 TB28 No kanamycin resistance 07/29
S5 K MG4 Strain needs to be grown on media with [NaCl]>=0.5%, preferably 1%NaCl 07/29
S6 DH5α DH5α E. Coli strain for chimically competent (Pasteur) 07/30
S7 A DH5α(g3p) DH5α E. Coli strain with g3p in plasmid, Amp resistance 07/30
S8 K DH5α(pSB2K3) DH5α E. Coli strain with pSB2K3 plasmid, Kan resistance 08/03
S9 A DH5α(BBa_J61002) DH5α E. Coli strain with BBa_J61002 in plasmid, Amp resistance 08/03
S10 K DH5α(BBa_J32015) DH5α E. Coli strain with BBa_J32015 in plasmid, Kan resistance 08/03
S11 K DH5α(BBa_B0014) DH5α E. Coli strain with BBa_B0014 in plasmid, Kan resistance 08/03
S12 K DH5α(BBa_I14032) DH5α E. Coli strain with BBa_I14032 in plasmid, Kan resistance 08/03
S13 A DH5α(BBa_K103006) DH5α E. Coli strain with BBa_K103006 in plasmid, Amp resistance 08/03
S14 A DH5α(BBa_R0040) DH5α E. Coli strain with BBa_R0040 in plasmid, Amp resistance 08/03
S15 K DH5α(BBa_10500) DH5α E. Coli strain with BBa_10500 in plasmid, Kan resistance 08/03
S16 K DH5α(ΔN-Syn1A) DH5α E. Coli strain with ΔN-Syn1A in plasmid, Kan resistance 08/03
S17 A DH5α(His6-SNAP25) DH5α E. Coli strain with His6-SNAP25 in plasmid, Amp resistance 08/03
S18 A DH5α(Vamp2-His6) DH5α E. Coli strain with Vamp2-His6 in plasmid, Amp resistance 08/03
S19 A DH5α(puc19) DH5α E. Coli strain with puc19 plasmid, Amp resistance 07/30
S20 K ΔfecA Keio Collection. JW4251. 53 C3. 08/04
S21 K ΔtolA Keio Collection. JN0729. 61 C12. 08/04
S22 K ΔtolR Keio Collection. JN0728.61 B12. 08/04
S23 K ΔtolQ Keio Collection. JN0727. 61 A12. 08/04
S24 K ΔOmpA Keio Collection. JN0940. 8 E4. 08/04
S25 K ΔtolB Keio Collection. JWS100. 67 C7. 08/04
S26 K ΔhlyE Keio Collection. JN5181. 75 F9. 08/04
S27 T FF64 MG 1655, F, tet resistance 08/04
S28 NEC 280 Hfr 3000 08/04
S29 A DH5α(pSB1A3) DH5α E. Coli strain with pSB1A3 plasmid, Amp resistance 08/06
S30 A DH5α(BBa_K136050) DH5α E. Coli strain with BBa_K136050 in plasmid, S113 iGEM 2008, Amp resistance 08/06
S31 A DH5α(BBa_J24679) DH5α E. Coli strain with BBa_K24679 in plasmid, Amp resistance 08/06
S32 Tri Strain Ming Strain : RpoH-RFP-iBpA, RpoS-YFP-ying, RpoD-CFP-pZ 08/09
S33 A DH5α(BBa_K103006) DH5α E. Coli strain with BBa_K103006 in plasmid, Amp resistance 08/14
S34 K DH5α(pSB2K3) DH5α E. Coli strain with pSB2K3 plasmid, Kan resistance 08/14
S35 AA93 ΔfecA 08/15
S36 C FecIR mutant FecR constitutif 08/15
S37 C FecIR wt ... 08/15
S38 A FecA lacZ ... 08/15

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