Team:Paris/Production testing delay

From 2009.igem.org

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== Delay ==
== Delay ==
 +
 +
We separate the construction on two plasmids :
 +
 +
 +
[[Team:Paris/Production_testing_delay#part1 | Part1 : testing delay phase 1]]
 +
 +
[[Team:Paris/Production_testing_delay#functional testing of the part 1 | Part1 : functional testing of the part 1]]
 +
 +
 +
 +
[[Team:Paris/Production_testing_delay#part2 | Part2 : testing delay phase 2]]
 +
 +
[[Team:Paris/Production_testing_delay#functional testing of the part 2 | Part2 : functional testing of the part 2]]
 +
===part1 ===
===part1 ===
-
[[Image:Paris_construc_3_delay1.jpg  |700px|center|plasmid = PSB3T5]]
+
[[Image:Paris_construc_3_delay1.jpg  |800px|center|plasmid = PSB3T5]]
 +
Time required : A lOT !!!!!
 +
Experiments ran :
 +
{|
 +
|- style="background: #0a3585; text-align: center; color:black; font-weight:bold; "
 +
|width="300px" ; bgcolor="#F8F8F8"| column 1
 +
|width="300px" ; bgcolor="#E0E3FE"| column 2
 +
|width="300px" ; bgcolor="#CBD1FD"| column 3
 +
|width="300px" ; bgcolor="#BCCDFD"| column 4
-
===part2 ===
+
|- style="background: #bebebe; text-align: center;"
 +
|'''PCR :'''
-
[[Image:Paris_construc_5_delay2.jpg  |700px|center|plasmid = ]]
+
TatABCE matrix :
 +
|'''PCR :'''
 +
|'''PCR :'''
 +
|'''PCR :'''
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''Verification on gel :'''
 +
ok
 +
|'''Verification on gel :'''
 +
-
 +
|'''Verification on gel :'''
 +
-
 +
|'''Verification on gel :'''
 +
 +
-
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''Purification on gel :'''
 +
 +
ok
 +
 +
|'''Purification on gel :'''
 +
 +
-
 +
 +
|'''Purification on gel :'''
 +
 +
-
 +
|'''Purification on gel :'''
 +
 +
-
 +
 +
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''digestion:'''
 +
 +
TatABCE
 +
 +
|'''digestion:'''
 +
|'''digestion:'''
 +
|'''digestion:'''
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''verification digestion:'''
 +
 +
ok
 +
 +
|'''verification digestion:'''
 +
 +
-
 +
 +
|'''verification digestion:'''
 +
 +
-
 +
|'''verification digestion:'''
 +
 +
-
 +
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''ligation:'''
 +
 +
TatABCE
 +
 +
|'''ligation:'''
 +
|'''ligation:'''
 +
|'''ligation:'''
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''PCR colo :'''
 +
 +
ok
 +
 +
|'''PCR colo :'''
 +
 +
-
 +
 +
|'''PCR colo :'''
 +
 +
-
 +
|'''PCR colo :'''
 +
 +
-
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''miniprep:'''
 +
 +
STOPPED
 +
 +
|'''miniprep:'''
 +
 +
-
 +
 +
|'''miniprep:'''
 +
 +
-
 +
 +
|'''miniprep:'''
 +
 +
-
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''sequencing :'''
 +
 +
-
 +
 +
|'''sequencing :'''
 +
 +
-
 +
 +
|'''sequencing :'''
 +
 +
-
 +
|'''sequencing :'''
 +
 +
-
 +
 +
|}
 +
 +
===functional testing of the part 1===
 +
 +
PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium :
 +
 +
 +
1% arabinose
 +
 +
1% glucose
 +
 +
LB pure
 +
 +
 +
<html>
 +
</div>
 +
<div id="paris_content_boxtop">
 +
</div>
 +
<div id="paris_content">
 +
</html>
 +
 +
===part2 ===
 +
 +
[[Image:Paris_construc_5_delay2.jpg  |800px|center|plasmid = ]]
Line 45: Line 208:
{|
{|
-
|- style="background: #0a3585; text-align: center; color:white; font-weight:bold; "
+
|- style="background: #0a3585; text-align: center; color:black; font-weight:bold; "
-
|width= 300px| row 1
+
|width="300px" ; bgcolor="#F8F8F8"| column 1
-
|width=300px| row 2
+
|width="300px" ; bgcolor="#E0E3FE"| column 2
-
|width=300px| row 3
+
|width="300px" ; bgcolor="#CBD1FD"| column 3
 +
|width="300px" ; bgcolor="#BCCDFD"| column 4
 +
 
|- style="background: #bebebe; text-align: center;"
|- style="background: #bebebe; text-align: center;"
|'''PCR :'''  
|'''PCR :'''  
-
 
+
TatABCE matrix :
-
 
+
|'''PCR :'''
|'''PCR :'''
|'''PCR :'''
|'''PCR :'''
-
 
+
|'''PCR :'''
|- style="background: #d8d8d8; text-align: center;"
|- style="background: #d8d8d8; text-align: center;"
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|'''Verification on gel :'''
|'''Verification on gel :'''
-
ok
+
-
|'''Verification on gel :'''
|'''Verification on gel :'''
-
ok
+
-
 +
|'''Verification on gel :'''
 +
 
 +
-
|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
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|'''Purification on gel :'''
|'''Purification on gel :'''
-
ok
+
-
|'''Purification on gel :'''
|'''Purification on gel :'''
-
ok
+
-
 +
|'''Purification on gel :'''
 +
 
 +
-
 +
 
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|'''digestion:'''  
|'''digestion:'''  
-
 
+
TatABCE
-
 
+
-
 
+
 +
|'''digestion:'''
|'''digestion:'''  
|'''digestion:'''  
|'''digestion:'''  
|'''digestion:'''  
Line 104: Line 274:
|'''verification digestion:'''
|'''verification digestion:'''
-
ok
+
-
|'''verification digestion:'''
|'''verification digestion:'''
-
ok
+
-
 +
|'''verification digestion:'''
 +
 
 +
-
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|'''ligation:'''  
|'''ligation:'''  
-
 
+
TatABCE
|'''ligation:'''  
|'''ligation:'''  
|'''ligation:'''  
|'''ligation:'''  
-
 
+
|'''ligation:'''
|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
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|'''PCR colo :'''
|'''PCR colo :'''
-
ok
+
-
|'''PCR colo :'''
|'''PCR colo :'''
-
ok
+
-
 +
|'''PCR colo :'''
 +
-
|- style="background: #d8d8d8; text-align: center;"
|- style="background: #d8d8d8; text-align: center;"
|'''miniprep:'''
|'''miniprep:'''
-
clone :
+
STOPPED
|'''miniprep:'''
|'''miniprep:'''
-
clone :
+
-
|'''miniprep:'''
|'''miniprep:'''
-
clone :
+
-
 +
|'''miniprep:'''
 +
 +
-
|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
|'''sequencing :'''
|'''sequencing :'''
-
ok
+
-
|'''sequencing :'''
|'''sequencing :'''
-
ok
+
-
|'''sequencing :'''
|'''sequencing :'''
-
ok
+
-
 +
|'''sequencing :'''
 +
 
 +
-
|}
|}
 +
 +
===functional testing of the part 2===
 +
 +
Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media :
 +
 +
 +
0,1% IPTG .
 +
 +
1% glucose.
 +
 +
LB pure.
 +
 +
 +
 +
[[Team:Paris/Production_testing_delay#top | return to the top]]

Latest revision as of 14:43, 18 October 2009

iGEM > Paris > Production > Testing > delay

Contents

WetLab

Main - Addressing - Production - reception -
Production

Delay

We separate the construction on two plasmids :


Part1 : testing delay phase 1

Part1 : functional testing of the part 1


Part2 : testing delay phase 2

Part2 : functional testing of the part 2


part1

plasmid = PSB3T5



Time required : A lOT !!!!!

Experiments ran :


column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-

functional testing of the part 1

PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium :


1% arabinose

1% glucose

LB pure


part2

plasmid =


Time required : A lOT !!!!!

Experiments ran :

column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-

functional testing of the part 2

Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media :


0,1% IPTG .

1% glucose.

LB pure.


return to the top

Retrieved from "http://2009.igem.org/Team:Paris/Production_testing_delay"