Team:Paris/Production testing delay

From 2009.igem.org

(Difference between revisions)
(→part1)
(→Delay)
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===part2 ===
===part2 ===
-
[[Image:Paris_construc_5_delay2.jpg  |700px|center|plasmid = ]]
+
[[Image:Paris_construc_5_delay2.jpg  |800px|center|plasmid = ]]
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Experiments ran :
Experiments ran :
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{|
 +
|- style="background: #0a3585; text-align: center; color:black; font-weight:bold; "
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|width="300px" ; bgcolor="#F8F8F8"| column 1
 +
|width="300px" ; bgcolor="#E0E3FE"| column 2
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|width="300px" ; bgcolor="#CBD1FD"| column 3
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|width="300px" ; bgcolor="#BCCDFD"| column 4
 +
 +
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|- style="background: #bebebe; text-align: center;"
 +
|'''PCR :'''
 +
 +
TatABCE matrix :
 +
 +
|'''PCR :'''
 +
|'''PCR :'''
 +
|'''PCR :'''
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''Verification on gel :'''
 +
 +
ok
 +
 +
|'''Verification on gel :'''
 +
 +
-
 +
 +
|'''Verification on gel :'''
 +
 +
-
 +
|'''Verification on gel :'''
 +
 +
-
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''Purification on gel :'''
 +
 +
ok
 +
 +
|'''Purification on gel :'''
 +
 +
-
 +
 +
|'''Purification on gel :'''
 +
 +
-
 +
|'''Purification on gel :'''
 +
 +
-
 +
 +
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''digestion:'''
 +
 +
TatABCE
 +
 +
|'''digestion:'''
 +
|'''digestion:'''
 +
|'''digestion:'''
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''verification digestion:'''
 +
 +
ok
 +
 +
|'''verification digestion:'''
 +
 +
-
 +
 +
|'''verification digestion:'''
 +
 +
-
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|'''verification digestion:'''
 +
 +
-
 +
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''ligation:'''
 +
 +
TatABCE
 +
 +
|'''ligation:'''
 +
|'''ligation:'''
 +
|'''ligation:'''
 +
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''PCR colo :'''
 +
 +
ok
 +
 +
|'''PCR colo :'''
 +
 +
-
 +
 +
|'''PCR colo :'''
 +
 +
-
 +
|'''PCR colo :'''
 +
 +
-
 +
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''miniprep:'''
 +
 +
STOPPED
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|'''miniprep:'''
 +
 +
-
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|'''miniprep:'''
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-
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|'''miniprep:'''
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 +
-
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|- style="background: #bebebe; text-align: center;" 
 +
|'''sequencing :'''
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-
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|'''sequencing :'''
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 +
-
 +
 +
|'''sequencing :'''
 +
 +
-
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|'''sequencing :'''
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 +
-
 +
 +
|}
[[Team:Paris/Production_testing_delay#top | return to the top]]
[[Team:Paris/Production_testing_delay#top | return to the top]]

Revision as of 14:30, 18 October 2009

iGEM > Paris > Production > Testing > delay

Contents

WetLab

Main - Addressing - Production - reception -
Production

Delay

We separate the construction on two plasmids :


Part1 : testing delay phase 1


Part2 : testing delay phase 2


part1

plasmid = PSB3T5



Time required : A lOT !!!!!

Experiments ran :


column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-

part2

plasmid =


Time required : A lOT !!!!!

Experiments ran :

column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-


return to the top

Retrieved from "http://2009.igem.org/Team:Paris/Production_testing_delay"