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Team:Paris/Protocols
From 2009.igem.org
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===3. Culture protocols=== | ===3. Culture protocols=== | ||
* Over Night | * Over Night | ||
| - | * | + | *[[Team:Paris/Protocols_Culture#Bacterial transformation | Bacterial transformation]] |
* [[Team:Paris/Protocols_Culture#Competent bacterias | Competent bacterias]] | * [[Team:Paris/Protocols_Culture#Competent bacterias | Competent bacterias]] | ||
**[[team:Paris/Protocols_Culture#CaCl2a|CaCl<sub>2</sub> (iGEM Paris2007)]] | **[[team:Paris/Protocols_Culture#CaCl2a|CaCl<sub>2</sub> (iGEM Paris2007)]] | ||
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**[[team:Paris/Protocols_Culture#RbCl|RbCl]] | **[[team:Paris/Protocols_Culture#RbCl|RbCl]] | ||
* [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]] | * [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]] | ||
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===4. Molecular biology=== | ===4. Molecular biology=== | ||
* PCR | * PCR | ||
Revision as of 12:46, 10 August 2009
Contents |
Protocols
Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.
Summary
1. Microscopy
2. Adapted Protocols
- DNA extraction
- Mini prep
- Gel/PCR Purification
3. Culture protocols
4. Molecular biology
- PCR
- Gels
- Digestion
- Ligation
- Protocol to make competent bacteria
- Protocole PCR quick load Taq mix
- [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method]
- Adaptation of PureYield™ Plasmid Miniprep System
- Protocol for bacterial transformation
A.2. iGEM paris 2008 protocols link
content :
- Electrophoresis
- Concentration of the Miniprep or the Midiprep
- Amplification of promoters.
- PCR Screening
- Protocol to make competent bacteria
A.3. iGEM paris 2007 protocols link
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
content :
- Growing_bacteria_in_liquid_medium
- Preparing growth media
- exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
- Chemical transformation
- Glycerol Stock
- Recombineering/Lambda red-mediated gene replacement
- Miniprep
- Fluorescent single cells visualisation
- Digestion reactions
