Team:TUDelft/14 August 2009

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='''14 August 2009'''=
='''14 August 2009'''=
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===Tim Weenink===
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First did ligation of !E and !F with the parts that were not excised from gel.
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{| border="1" align="center"
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| Component for !E || volume (uL)
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|- align="center"
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| *S PCR (upstream) || 2
 +
|- align="center"
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| !C PCR (downstream) || 2
 +
|- align="center"
 +
| pSB1AK3 from !A assembly || 2
 +
|- align="center"
 +
| T4 ligase buffer || 2
 +
|- align="center"
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| T4 ligase || 1
 +
|- align="center"
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| H2O || 11
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|}
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 +
{| border="1" align="center"
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| Component for !F || volume (uL)
 +
|- align="center"
 +
| !B PCR (upstream) || 2
 +
|- align="center"
 +
| !D PCR (downstream) || 2
 +
|- align="center"
 +
| pSB1AK3 from !A assembly || 2
 +
|- align="center"
 +
| T4 ligase buffer || 2
 +
|- align="center"
 +
| T4 ligase || 1
 +
|- align="center"
 +
| H2O || 11
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|}
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 +
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 12:47, 14 August 2009

Lab Notebook

July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

14 August 2009

Tim Weenink

First did ligation of !E and !F with the parts that were not excised from gel.

Component for !E volume (uL)
*S PCR (upstream) 2
 !C PCR (downstream) 2
pSB1AK3 from !A assembly 2
T4 ligase buffer 2
T4 ligase 1
H2O 11
Component for !F volume (uL)
 !B PCR (upstream) 2
 !D PCR (downstream) 2
pSB1AK3 from !A assembly 2
T4 ligase buffer 2
T4 ligase 1
H2O 11


Retrieved from "http://2009.igem.org/Team:TUDelft/14_August_2009"