Team:TUDelft/14 August 2009

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Revision as of 12:47, 14 August 2009 by Tim Weenink (Talk | contribs)

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14 August 2009

Tim Weenink

First did ligation of !E and !F with the parts that were not excised from gel.

Component for !E volume (uL)
*S PCR (upstream) 2
 !C PCR (downstream) 2
pSB1AK3 from !A assembly 2
T4 ligase buffer 2
T4 ligase 1
H2O 11
Component for !F volume (uL)
 !B PCR (upstream) 2
 !D PCR (downstream) 2
pSB1AK3 from !A assembly 2
T4 ligase buffer 2
T4 ligase 1
H2O 11


Retrieved from "http://2009.igem.org/Team:TUDelft/14_August_2009"