Team:TUDelft/17 August 2009
From 2009.igem.org
Lab Notebook
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17 August 2009
Calin
The backup trbK_KO pate has 11 colonies. The Plate 3L oriTR_KO -L-ara +PCR also had 8 colonies. No growth on any other plates. Plates with growth put in fridge.
Ran colony PCR on 5 Colonies: Did colony PCR for 2 different trbK_KO colonies and also made 5mL culture tubes for them. Also did colony PCR on the oriTR control and 1 R751+pKD46+trbK_mod_S and 1 R751+pKD46+oriTR_mod_S for reference.
Made 100uM and 40uM stock of verification primers.
Made 1 L LB agar.
Made 10mL of TRIMETHOPRIM stock.
Tim Vos helped me transform CA into R751 Electrocompetent cells. Both plate and 5mL cultures made.
Ran a 1% and a 2% gel with all 5 colony PCR samples.
Daniel made 10 TRI, 4 CAM, and 6 AMP plates.
I made a further 15 AMP + CAM plates.