Team:TUDelft/18 September 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{Template:TUDelftiGEM2009}} {{Template:TUDelftiGEM2009_LabNotebook}} ='''18 September 2009'''= {{Template:TUDelftiGEM2009_end}})
(18 September 2009)
Line 4: Line 4:
='''18 September 2009'''=
='''18 September 2009'''=
-
 
+
We found why the sequencing failed last time. First of all Tim gave wrong primers secondly my stupidity I added both primers in same sample misinterpreting what Tim meant for two separate same larger DNA samples. So today I sent samples again for sequencing.
 +
I got nice colonies with the lacI generator attached. None of the colonies are now glowing. Hence I think my assembly worked.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 00:22, 22 October 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

18 September 2009

We found why the sequencing failed last time. First of all Tim gave wrong primers secondly my stupidity I added both primers in same sample misinterpreting what Tim meant for two separate same larger DNA samples. So today I sent samples again for sequencing. I got nice colonies with the lacI generator attached. None of the colonies are now glowing. Hence I think my assembly worked.