Team:TUDelft/19 August 2009

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19 August 2009

Tim Weenink

After yesterdays horrible discovery that the I-SceI Homing Endonuclease we got from MIT was actually T4 DNA ligase we had to adjust our cloning strategy. The more primitive part without promoter was found to be the correct one, except that is was assembled with part !A instead of p(LacI). So we did that assembly again today. With p(LacI) from the delay team upstream, *T1 (downstream) and backbone pSB1C3 (also from the delay team). Both RbCl chemically comp and EC electrocomp cells were transformed.


Saeed

Saeed19082009wiki.jpg

lane Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] 2953
2  !Fr1n upstream 1162 ok
3  !Fr1n downstream 311 ok
4 destination plasmid 1162 ok