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Team:TUDelft/20 August 2009
From 2009.igem.org
Lab Notebook
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20 August 2009
Meeting with Groningen's and Amsterdam's team (see pictures)
Tim Weenink
We got colonies on yesterdays transformations. RbCl plates had about 20 colonies each, while Electrocompetent cells were hundreds of colonies. Finally an oldfashioned highly efficient transformation. I suspect transformations with larger parts (>1kb) work better.
I inoculated some of the colonies. These are the names of the samples:
R/E stands for RbCl/Electrocompetent (resp) 1C3 stands for the used backbone (pSB1C3) α ß stands for plate numbers 1,2 stands for colony numbers
- !SIIR1C3α1
- !SIIR1C3α2
- !SIIR1C3ß1
- !SIIR1C3ß2
- !SIIE1C3α1
- !SIIE1C3α2
- !SIIE1C3ß1
- !SIIE1C3ß2
Daniel
Growth in assemblies 1A, 2A, 3A, 4A and 5A. 6A failbut we found a biobrick with the same structure and we have it in plates already!!
Miniprep of succeed assemblies:
| Assembly | [DNA] ng/uL |
| 1A | 20.5 |
| 2A I | 18.7 |
| 2A II | 26.5 |
| 2A III | 25.5 |
| 2A IV | 32.2 |
| 3A | 13 |
| 4A I | 13.9 |
| 4A II | 8.7 |
| 5A | 4.9 |
Calin
Checked KO plates. Some small colonies. Left plates in incubator.
