Team:TUDelft/24 August 2009

From 2009.igem.org

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'''Right Gel (2%)'''
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'''Left Gel (2%)'''
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{| border="1" align="center"
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| Well || Assembly || Expected Plasmid Size || Status
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|- align="center"
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| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 2 || col PCR 1 || 1616  || <font color=red>&#10006;</font>
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|- align="center"
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| 3 || col PCR 2 || 1616  || <font color=red>&#10006;</font>
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|- align="center"
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| 4 || col PCR 3 || 1616  || <font color=red>&#10006;</font>
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|- align="center"
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| 5 || col PCR 4 ||  1616 || <font color=red>&#10006;</font>
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|- align="center"
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| 6 || col PCR 5 || 1616  || <font color=red>&#10006;</font>
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|- align="center"
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| 7 || col PCR 6 || 1616  ||  <font color=red>&#10006;</font>
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|- align="center"
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| 8 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
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|}
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'''Right Gel (1%)'''
{| border="1" align="center"
{| border="1" align="center"
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| 6 || 1A || 1076 || ?
| 6 || 1A || 1076 || ?
|- align="center"
|- align="center"
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| 7 || PCR 1 ||   ||
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| 7 || col PCR 1 || 1616 || <font color=red>&#10006;</font>
|- align="center"
|- align="center"
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| 8 || PCR 2 ||   ||
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| 8 || col PCR 2 || 1616 || <font color=red>&#10006;</font>
|- align="center"
|- align="center"
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| 9 || PCR 3 ||   ||
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| 9 || col PCR 3 || 1616 || <font color=red>&#10006;</font>
|- align="center"
|- align="center"
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| 10 || PCR 4 ||   ||
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| 10 || col PCR 4 || 1616  || <font color=red>&#10006;</font>
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|- align="center"
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| 11 || PCR 5 ||   ||
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| 11 || col PCR 5 || 1616  || <font color=red>&#10006;</font>
|- align="center"
|- align="center"
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| 12 || PCR 6 ||   ||  
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| 12 || col PCR 6 || 1616  || <font color=red>&#10006;</font>
|}
|}

Revision as of 12:27, 19 October 2009

Lab Notebook

July
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24 August 2009

Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 colonies

Daniel

Digestion of assemblies in order to do the second round of assembly. Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR

Gel240809.jpg


Left Gel (2%)

Well Assembly Expected Plasmid Size Status
1 DNA Ladder ✔
2 col PCR 1 1616 ✖
3 col PCR 2 1616 ✖
4 col PCR 3 1616 ✖
5 col PCR 4 1616 ✖
6 col PCR 5 1616 ✖
7 col PCR 6 1616 ✖
8 DNA Ladder ✔

Right Gel (1%)

Well Assembly Expected Plasmid Size Status
1 DNA Ladder ✔
2 4A 824  ?
3 5A 1040 ✔
4 2A 214  ?
5 3A 183  ?
6 1A 1076  ?
7 col PCR 1 1616 ✖
8 col PCR 2 1616 ✖
9 col PCR 3 1616 ✖
10 col PCR 4 1616 ✖
11 col PCR 5 1616 ✖
12 col PCR 6 1616 ✖

Prepare IPTG 1M for further induction. I tried to do an experiment with 1A which is GFP under control of PLacI however the culture glows even though there is no IPTG induction.

We got the oligo for the two locks and keys I designed.

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